Anti-MTMR14 antibody (ab76952)
Key features and details
- Rabbit polyclonal to MTMR14
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MTMR14 antibody
See all MTMR14 primary antibodies -
Description
Rabbit polyclonal to MTMR14 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human MTMR14 aa 600 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab92660) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HEK293; HepG2; A549.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications CowApplication Abreviews Notes WB Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 72 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Lipid phosphatase which efficiently dephosphorylates phosphatidylinositol 3-phosphate (PtdIns3P) and PtdIns(3,5)P2; inactive toward PtdIns4P, PtdIns(3,4)P2, PtdIns(4,5)P2 and PtdIns(3,4,5)P3. -
Tissue specificity
Expressed in various tissues, including heart, skeletal muscle, placenta, liver, lung, kidney and pancreas. -
Involvement in disease
Defects in MTMR14 may be a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. -
Cellular localization
Cytoplasm. Found in reticular structures and plasma membrane ruffles. Concentrated near the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 514596 Cow
- Entrez Gene: 64419 Human
- Omim: 611089 Human
- SwissProt: Q8NCE2 Human
- Unigene: 475382 Human
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Alternative names
- C3orf29 antibody
- Egg derived tyrosine phosphatase homolog antibody
- FLJ11546 antibody
see all
Images
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Western blot - Anti-MTMR14 antibody (ab76952)All lanes : Anti-MTMR14 antibody (ab76952) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
MTMR14 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab76952 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-MTMR14 antibody (ab76952)All lanes : Anti-MTMR14 antibody (ab76952) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
MTMR14 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
