Mouse Milk Fat Globule 1 Matched Antibody Pair Kit (ab216785)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 48.9 pg/ml - 6000 pg/ml
Overview
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Product name
Mouse Milk Fat Globule 1 Matched Antibody Pair Kit
See all Milk Fat Globule 1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.18 pg/ml -
Range
48.9 pg/ml - 6000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Milk Fat Globule 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Milk Fat Globule 1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208981. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Milk Fat Globule 1 Capture Antibody 2 x 50µg 1 x 50µg Mouse Milk Fat Globule 1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Milk Fat Globule 1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
Medin is the main constituent of aortic medial amyloid. -
Tissue specificity
Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas. -
Sequence similarities
Contains 1 EGF-like domain.
Contains 2 F5/8 type C domains. -
Post-translational
modificationsMedin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273. -
Cellular localization
Membrane. Secreted. - Information by UniProt
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Alternative names
- BA46
- Breast epithelial antigen BA46
- EDIL1
see all -
Database links
- Entrez Gene: 17304 Mouse
- SwissProt: P21956 Mouse
Images
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Mouse Milk Fat Globule 1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Milk Fat Globule 1 Matched Antibody Pair Kit (ab216785)To learn more about the advantages of recombinant antibodies see here.
