Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL33] to SLC1A5/ASCT2 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free
    See all SLC1A5/ASCT2 primary antibodies

  • Description

    Rabbit monoclonal [CAL33] to SLC1A5/ASCT2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon carcinoma and lung carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate. ICC/IF: Jurkat and HeLa cells. Flow Cyt: Jurkat and HeLa cells. IP: Jurkat and HeLa whole cell lysate.

  • General notes

    ab251591 is the carrier-free version of ab237704 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251591 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as SLC1A5

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling SLC1A5/ASCT2 with ab237704 at 1/1600 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human colon carcinoma is observed. The section was incubated with ab237704 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling SLC1A5/ASCT2 with ab237704 at 1/1600 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human lung carcinoma is observed. The section was incubated with ab237704 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for SLC1A5/ASCT2 using ab237704 at 0.5 µg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

  • Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)
    Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)

    Immunofluorescent analysis of 100% methanol fixed Jurkat (human T cell leukemia T lymphocyte) cells labeling SLC1A5/ASCT2 with ab237704 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Jurkat cells. The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

  • Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)
    Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)

    Immunofluorescent analysis of 100% methanol fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling SLC1A5/ASCT2 with ab237704 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in HeLa cells. The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

  • Flow Cytometry - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)
    Flow Cytometry - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling SLC1A5/ASCT2 with ab237704 at 1/400 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

  • Flow Cytometry - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)
    Flow Cytometry - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte) cell line labeling SLC1A5/ASCT2 with ab237704 at 1/40 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

  • Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)
    Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)

    SLC1A5/ASCT2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab237704 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237704 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
    Lane 1: HeLa whole cell lysate 10 µg (Input).
    Lane 2: ab237704 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237704 in HeLa whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 1 second.

    ASCT2 (SLC1A5) can form dimers or trimers based on literature. (PMID: 23756778).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

  • Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)
    Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)

    SLC1A5/ASCT2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab237704 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237704 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
    Lane 1: Jurkat whole cell lysate 10 µg (Input).
    Lane 2: ab237704 IP in Jurkat whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237704 in Jurkat whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    ASCT2 (SLC1A5) can form dimers or trimers based on literature. (PMID: 23756778).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

  • Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)
    Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (ab251591)

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