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Anti-RPA32/RPA2 (phospho T21) antibody (ab61065)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-RPA32/RPA2 (phospho T21) antibody (ab61065)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to RPA32/RPA2 (phospho T21)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RPA32/RPA2 (phospho T21) antibody
    See all RPA32/RPA2 primary antibodies
  • Description

    Rabbit polyclonal to RPA32/RPA2 (phospho T21)
  • Host species

    Rabbit
  • Specificity

    ab61065 detects endogenous levels of RPA32/RFA2 only when phosphorylated at threonine 21.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RPA32/RPA2. The exact sequence is proprietary. Synthesized phosphopeptide derived from human RPA32/RFA2 around the phosphorylation site of threonine 21(G-Y-TP-Q-S).
    Database link: P15927

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab61065 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Images

  • Western blot - Anti-RPA32/RPA2 (phospho T21) antibody (ab61065)
    Western blot - Anti-RPA32/RPA2 (phospho T21) antibody (ab61065)
    All lanes : Anti-RPA32/RPA2 (phospho T21) antibody (ab61065) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates treated with Adriamycin 0.5 µg/ml 24 hours
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates treated with Adriamycin 0.5 µg/ml 24 hours with immunizing phosphopeptide

    Developed using the ECL technique.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho T21) antibody (ab61065)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 (phospho T21) antibody (ab61065)

    Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab61065 at 1/50 dilution.

    Left panel: Without immunizing phosphopeptide.

    Right panel: Plus immunizing phosphopeptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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