Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] (ab254255)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22455-57] to SerpinB3/SCCA + SerpinB4/SCCA-2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57]

  • Description

    Rabbit monoclonal [EPR22455-57] to SerpinB3/SCCA + SerpinB4/SCCA-2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 whole cell lysate. Human fetal lung tissue lysate. IHC-P: Human pancreatic carcinoma and ovarian cancer tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [EPR22455-57] (ab254255)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [EPR22455-57] (ab254255)

    Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma and the paired adjacent pancreas labeling SerpinB3/SCCA with ab254255 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreatic carcinoma (panel A) and no staining on the paired adjacent pancreas (panel B, PMID: 24759783) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254255 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-SerpinB3/SCCA antibody [EPR22455-57] (ab254255)
    Western blot - Anti-SerpinB3/SCCA antibody [EPR22455-57] (ab254255)
    All lanes : Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] (ab254255) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 3 : Human fetal lung tissue lysate
    Lane 4 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 3 minutes


    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:24517394).

    Negative control: Human liver (PMID:28611447).

    HepG2 as a low serpinB expressing cell line.

    This blot was developed using a higher sensitivity ECL substrate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [EPR22455-57] (ab254255)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [EPR22455-57] (ab254255)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer labeling SerpinB3/SCCA with ab254255 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on ovarian cancer (PMID: 23185467) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254255 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-SerpinB3/SCCA antibody [EPR22455-57] (ab254255)
    Western blot - Anti-SerpinB3/SCCA antibody [EPR22455-57] (ab254255)
    All lanes : Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] (ab254255) at 1/1000 dilution

    Lane 1 : Recombinant Human Serpin B3/SSCA1 Protein 20 ng
    Lane 2 : Recombinant Human Serpin B4/SCCA-2 Protein 20 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3.25 secs.

  • Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] (ab254255)
    Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] (ab254255)

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