Lactose Assay Kit (ab83384)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Milk, Other biological fluids
Overview
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Product name
Lactose Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Milk, Other biological fluids -
Assay type
Quantitative -
Assay time
1h 00m -
Product overview
In Abcam's Lactose Assay Kit Lactose is hydrolyzed to glucose and galactose. The galactose is subsequently oxidized generating color (OD570nm) and fluorescence (Ex/Em 535/587 nm). Free galactose can be corrected by a background control in the absence of lactase. The Lactose Assay Kit provides a simple, convenient, and sensitive means for direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). Pretreatment of samples is not required. The kit can be used as a high throughput assay.
Visit our FAQs page for tips and troubleshooting. -
Notes
Lactose (C12H22O11 FW: 342.3) is an important naturally occurred disaccharide, consisting of one galactose and one glucose. Milk contains ~2-8% lactose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests HRP (Lyophilized) Purple 1 vial Lactase (Lyophilized) Blue 1 vial Lactose Assay Buffer WM 1 x 25ml Lactose Enzyme Mix (Lyophilized) Green 1 vial Lactose Standard (100 nmol/ul) Yellow 1 x 100µl Probe (DMSO solution) Red 1 x 200µl -
Research areas
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Relevance
Lactose (C12H22O11 FW: 342.3) is an important naturally occurring disaccharide along with sucrose and maltose. Lactose makes up around 2-8% of milk. It consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage. -
Alternative names
- Milk sugar
Images
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Functional Studies - Lactose Assay Kit (ab83384)Lactose Standard Curve: Assays were performed following the kit instructions. The kit detected galactose and lactose equally. In the absence of Lactase, the kit detected galactose, but not lactose.