Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR443] to ARF1 + ARF3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-ARF1 + ARF3 antibody [EPR443]
    See all ARF1+ARF3 primary antibodies

  • Description

    Rabbit monoclonal [EPR443] to ARF1 + ARF3

  • Host species

    Rabbit

  • Specificity

    The immunogen used to raise this antibody shares 100% homology with human ARF3, 96% homology with ARF4 and ARF5, and 88% homology with ARF6. Cross-reactivity with the latter three proteins have not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney, Human fetal brain and MCF7 lysates; Human breast ductal carcinoma, and Human colonic adenocarcinoma tissues This antibody gave a positive result when used in the following methanol fixed cell lines: CaCo2

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    All lanes : Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/50000 dilution

    Lane 1 : Full length human ARF-1 recombinant protein
    Lane 2 : Full length human ARF-3 recombinant protein

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM /TBST

    Human ARF-1 recombinant protein, (Cat#:ab123538 )
    Human ARF-3 recombinant protein, (Cat#:ab113581 )

  • Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

    ICC/IF image of ab108347 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108347 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    All lanes : Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : MCF7 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

    Immunohistochemical analysis of ARF1 in paraffin embedded Human breast ductal carcinoma tissue, using ab108347 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

    Immunohistochemical analysis of ARF1 in paraffin embedded Human colonic adenocarcinoma tissue, using ab108347 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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