Anti-KMT1E / SETDB1 antibody (ab228984)
Key features and details
- Rabbit polyclonal to KMT1E / SETDB1
- Suitable for: WB, IP, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KMT1E / SETDB1 antibody
See all KMT1E / SETDB1 primary antibodies -
Description
Rabbit polyclonal to KMT1E / SETDB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ChIPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human KMT1E/ SETDB1 (internal sequence). The exact sequence is proprietary.
Database link: Q15047 -
Positive control
- ChIP: HeLa chromatin extract. WB: U-87 MG whole cell lysate. IP: HeLa nuclear extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KMT1E / SETDB1 antibody (ab228984)Anti-KMT1E / SETDB1 antibody (ab228984) at 1/2000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 143 kDa5% SDS-PAGE
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ChIP - Anti-KMT1E / SETDB1 antibody (ab228984)Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract and 5 μg of either control rabbit IgG or ab228984. The precipitated DNA was detected by PCR with a primer set targeting to P53P2.
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Immunoprecipitation - Anti-KMT1E / SETDB1 antibody (ab228984)ab228984 immunoprecipitates SETDB1 protein in IP experiments.
IP samples: HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract.Lane 1: Control IP with 4 μg of preimmune Rabbit IgG.
Lane 2: Immunoprecipitation of SETDB1 protein by 4 μg ab228984.
The immunoprecipitated SETDB1 protein was detected by ab228984 at 1/1000 dilution.5 % SDS-PAGE
