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Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)

Key features and details

  • Mouse monoclonal [5H6A12] to KMT1E / SETDB1
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Mouse, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-KMT1E / SETDB1 antibody [5H6A12]
    See all KMT1E / SETDB1 primary antibodies

  • Description

    Mouse monoclonal [5H6A12] to KMT1E / SETDB1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Purified recombinant fragment of human SETDB1 expressed in E. Coli.

  • Positive control

    • MCF-7,T47D, HEK293, JURKAT, NIH/3T3, F9, RAW246.7 and COS-7 cell lysates.

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR223392-13 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)
    Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)
    All lanes : Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225) at 1/500 dilution

    Lane 1 : MCF-7
    Lane 2 : T47D
    Lane 3 : HEK293
    Lane 4 : JURKAT
    Lane 5 : NIH/3T3
    Lane 6 : F9
    Lane 7 : RAW264.7
    Lane 8 : COS-7

    Predicted band size: 180 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)
    Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)
    Immunofluoresence detection of SETDB1 in LOVO cells using ab107225 (green). Actin fillaments have been labeled with Alexa Fluor-555 phalloidin (red).
  • Flow Cytometry - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)
    Flow Cytometry - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)
    Overlay histogram showing Ramos cells stained with ab107225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107225, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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