Anti-KMT6 / EZH2 (phospho S21) antibody (ab84989)
Key features and details
- Rabbit polyclonal to KMT6 / EZH2 (phospho S21)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KMT6 / EZH2 (phospho S21) antibody
See all KMT6 / EZH2 primary antibodies -
Description
Rabbit polyclonal to KMT6 / EZH2 (phospho S21) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Phosphorylated synthetic peptide surrounding Serine 21 of human KMT6/ EZH2 (NP_004447.2)
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Positive control
- IHC-P: Human Breast Carcinoma, Colon Carcinoma and lung carcinoma tissues; Mouse Teratoma. ICC/IF: HepG2 cells.
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General notes
Concentration is optimized for IHC and not determined
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for KMT6 / EZH2 (phospho S21) using ab84989 at 1/250 dilution in immunohistochemical analysis. DAB staining.
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ab84989 at 1/250 dilution staining KMT6 / EZH2 in serial sections of human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right). -
ab84989 at 1/250 dilution staining KMT6 / EZH2 in serial sections of mouse teratoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right). -
ab84989 at 1/250 dilution staining KMT6 / EZH2 in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
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ICC/IF image of ab84989 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84989, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.