Anti-Ctip2 antibody (ab70453)
Key features and details
- Rabbit polyclonal to Ctip2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ctip2 antibody
See all Ctip2 primary antibodies -
Description
Rabbit polyclonal to Ctip2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to a region between C terminal residues 850-894 of human Ctip2 (Swiss-Prot Q9C0K0).
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Positive control
- WB: Jurkat, MOLT-4 cells; IP:Jurkat; IHC: human prostate carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody (ab70453)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Ctip2 with ab70453 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Ctip2 antibody (ab70453)All lanes: 1mg nuclear extract from Jurkat cells analysed on a blot using ab70453 at 0.5µg/ml.
Lane 1: an alternate Ctip2 antibody used at 1 µg/mg lysate for IP.
Lane 2: The same alternate antibody used at 10 µg/mg lysate for IP.
Lane 3: ab70453 used at 1 µg/mg lysate for IP.
Lane 4: ab70453 at 10 µg/mg lysate for IP.
Normal rabbit IgG was used as a control for immunoprecipitation reactions. -
Western blot - Anti-Ctip2 antibody (ab70453)All lanes : Anti-Ctip2 antibody (ab70453) at 0.1 µg/ml
Lane 1 : Jurkat cell nuclear extract at 5 µg
Lane 2 : Jurkat cell nuclear extract at 50 µg
Predicted band size: 100 kDa
Observed band size: 120-130 kDa why is the actual band size different from the predicted?
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Western blot - Anti-Ctip2 antibody (ab70453)All lanes : Anti-Ctip2 antibody (ab70453) at 0.2 µg/ml
Lane 1 : L1210 whole cell lysate
Lane 2 : TCMK-1 whole cell lysate
Lane 3 : 4T1 whole cell lysate
Lane 4 : CT26.WT whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 100 kDa
Exposure time: 3 minutes
