Anti-PGP9.5 antibody (ab227104)
Key features and details
- Rabbit polyclonal to PGP9.5
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-PGP9.5 antibody
See all PGP9.5 primary antibodies -
Description
Rabbit polyclonal to PGP9.5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig -
Immunogen
Recombinant fragment within Human PGP9.5 (internal sequence). The exact sequence is proprietary.
Database link: P09936 -
Positive control
- WB: Rat brain extract; A549 and H1299 whole cell lysates; Mouse brain lysate. IHC-Fr: E13.5 rat brain tissue. IHC-P: Human oral cancer tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Paraffin-embedded human oral cancer tissue stained for PGP9.5 using ab227104 at 1/100 dilution in immunohistochemical analysis.
-
Anti-PGP9.5 antibody (ab227104) at 1/1000 dilution + Rat brain extract at 50 µg
Predicted band size: 24 kDa12% SDS-PAGE gel.
-
Frozen sectioned E13.5 rat brain tissue stained for PGP9.5 (green) using ab227104 at 1/500 dilution in immunohistochemical analysis.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody at 1/500 dilution.
Blue: DAPI.
-
All lanes : Anti-PGP9.5 antibody (ab227104) at 1/5000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 24 kDa12% SDS-PAGE gel.
-
Anti-PGP9.5 antibody (ab227104) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 24 kDa12% SDS-PAGE gel.