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Anti-DOCK180 antibody (ab97325)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-DOCK180 antibody (ab97325)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to DOCK180
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-DOCK180 antibody
    See all DOCK180 primary antibodies
  • Description

    Rabbit polyclonal to DOCK180
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human DOCK180 aa 1852-1865.
    Sequence:

    TTRKQASVDSGIVQ


    Database link: Q14185
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HEK-293T whole cell lysate. C8D30 , NIH/3T3 , Raw264.7 , C2C12 whole cell extracts. PC-12 whole cell extracts. ICC/IF: A431 cells IHC-P: Lung cancer.
  • General notes

    Previously labelled as DOCK1. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Phagocytosis
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Phagocytosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-DOCK180 antibody (ab97325)
    Western blot - Anti-DOCK180 antibody (ab97325)
    Anti-DOCK180 antibody (ab97325) at 1/1000 dilution + HEK-293T whole cell lysate at 30 µg

    Predicted band size: 215 kDa



    7.5% SDS Page
  • Immunocytochemistry/ Immunofluorescence - Anti-DOCK180 antibody (ab97325)
    Immunocytochemistry/ Immunofluorescence - Anti-DOCK180 antibody (ab97325)

    Immunofluorescence analysis of DOCK180 in methanol fixed A431 using ab97325 at a 1/200 dilution.

  • Western blot - Anti-DOCK180 antibody (ab97325)
    Western blot - Anti-DOCK180 antibody (ab97325)
    All lanes : Anti-DOCK180 antibody (ab97325) at 1/1000 dilution

    Lane 1 : C8D30 whole cell extract
    Lane 2 : NIH/3T3 whole cell extract
    Lane 3 : Raw264.7 whole cell extract
    Lane 4 : C2C12 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 215 kDa



    5% SDS-PAGE.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK180 antibody (ab97325)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK180 antibody (ab97325)

    Immunohistochemical analysis of DOCK180 in paraffin embedded Lung cancer using ab97325 at a 1/100 dilution.

  • Western blot - Anti-DOCK180 antibody (ab97325)
    Western blot - Anti-DOCK180 antibody (ab97325)
    Anti-DOCK180 antibody (ab97325) at 1/1000 dilution + PC-12 whole cell extract at 30 µg

    Predicted band size: 215 kDa



    5% SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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