Anti-DOCK180 antibody (ab97325)
Key features and details
- Rabbit polyclonal to DOCK180
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DOCK180 antibody
See all DOCK180 primary antibodies -
Description
Rabbit polyclonal to DOCK180 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human DOCK180 aa 1852-1865.
Sequence:TTRKQASVDSGIVQ
Database link: Q14185 -
Positive control
- WB: HEK-293T whole cell lysate. C8D30 , NIH/3T3 , Raw264.7 , C2C12 whole cell extracts. PC-12 whole cell extracts. ICC/IF: A431 cells IHC-P: Lung cancer.
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General notes
Previously labelled as DOCK1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-DOCK180 antibody (ab97325) at 1/1000 dilution + HEK-293T whole cell lysate at 30 µg
Predicted band size: 215 kDa
7.5% SDS Page -
Immunofluorescence analysis of DOCK180 in methanol fixed A431 using ab97325 at a 1/200 dilution.
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All lanes : Anti-DOCK180 antibody (ab97325) at 1/1000 dilution
Lane 1 : C8D30 whole cell extract
Lane 2 : NIH/3T3 whole cell extract
Lane 3 : Raw264.7 whole cell extract
Lane 4 : C2C12 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 215 kDa5% SDS-PAGE.
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Immunohistochemical analysis of DOCK180 in paraffin embedded Lung cancer using ab97325 at a 1/100 dilution.
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Anti-DOCK180 antibody (ab97325) at 1/1000 dilution + PC-12 whole cell extract at 30 µg
Predicted band size: 215 kDa5% SDS-PAGE