Anti-DOCK180 antibody (ab76927)
Key features and details
- Rabbit polyclonal to DOCK180
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DOCK180 antibody
See all DOCK180 primary antibodies -
Description
Rabbit polyclonal to DOCK180 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human DOCK180.
Database link: NP_001371.1 -
Positive control
- HepG2 whole cell lysate (ab7900) can be used as a positive control in WB. HeLa, 293T and NIH3T3 whole cell lysates.
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General notes
Previously labelled as DOCK1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab76927 was affinity purified using an epitope specific to DOCK180 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DOCK180 antibody (ab76927)All lanes : Anti-DOCK180 antibody (ab76927) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 215 kDa
Observed band size: 215 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-DOCK180 antibody (ab76927)Detection of DOCK180 by Western Blot of Immunprecipitate.
ab76927, at 1µg/ml, staining DOCK180 in HeLa whole cell lysate immunoprecipitated using ab76927 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
