Anti-HDAC2 antibody - C-terminal (ab227149)
Key features and details
- Rabbit polyclonal to HDAC2 - C-terminal
- Suitable for: ChIP, IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC2 antibody - C-terminal
See all HDAC2 primary antibodies -
Description
Rabbit polyclonal to HDAC2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human HDAC2 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q92769 -
Positive control
- ICC/IF: HeLa cells. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; C2C12 and Rat2 whole cell lysates. IP: HeLa whole cell lysate. ChIP: HEK-293T whole cell lysate/extract. IHC-P: Cal27 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HDAC2 antibody - C-terminal (ab227149)All lanes : Anti-HDAC2 antibody - C-terminal (ab227149) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extracts
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 4 : HepG2 (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
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ChIP - Anti-HDAC2 antibody - C-terminal (ab227149)ab227149 immunoprecipitates HDAC2 protein-DNA complex in ChIP experiments.
ChIP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract.
A: 5 μg preimmune rabbit IgG
B: 5 μg of ab227149
The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter. -
Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody - C-terminal (ab227149)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HDAC2 (green) using ab227149 at 1/500 dilution in ICC/IF. Alpha-tubulin filaments were labeled (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC2 antibody - C-terminal (ab227149)Paraffin embedded Cal27 (human) xenograft tissue stained for HDAC2 using ab227149 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-HDAC2 antibody - C-terminal (ab227149)HDAC2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate. Western blot was performed from the immunoprecipitate using ab227149 at 1/1000 dilution. Anti-rabbit IgG was used as a secondary reagent.
Lane 1: 40 μg HeLa whole cell lysate.
Lane 2: Control with 2 μg of preimmune rabbit IgG.
Lane 3: Immunoprecipitation of HDAC2 protein by 2 μg of ab227149.
7.5% SDS-PAGE gel.
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Western blot - Anti-HDAC2 antibody - C-terminal (ab227149)Anti-HDAC2 antibody - C-terminal (ab227149) at 1/1000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-HDAC2 antibody - C-terminal (ab227149)Anti-HDAC2 antibody - C-terminal (ab227149) at 1/1000 dilution + C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
