Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24097-105] to Decorin - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Decorin antibody [EPR24097-105] - BSA and Azide free
    See all Decorin primary antibodies

  • Description

    Rabbit monoclonal [EPR24097-105] to Decorin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human colon, uterus, pancreas and placenta tissue lysates; Mouse uterus and colon tissue lysates; Rat uterus and colon tissue lysates. IHC-P: Human colon, esophagus, bladder cancer and liver tissue. Flow cyt: MG-63 cells.

  • General notes

    ab277643 is the carrier-free version of ab277636. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277643 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    All lanes : Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

    Lane 1 : Human uterus tissue lysate
    Lane 2 : Human pancreas tissue lysate
    Lane 3 : Human placenta tissue lysate
    Lane 4 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 40 kDa
    Observed band size: 90-140 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab277636, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

    Lower expression tissue: liver (PMID:1611907).

    Exposure time: Lane 1: 8 seconds; Lanes 2-4: 48 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

    This data was developed using ab277636, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human colon (PMID:32824864). The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    All lanes : Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

    Lane 1 : MG-63 (human osteosarcoma fibroblast) whole cell lysate
    Lane 2 : Mouse uterus tissue lysate
    Lane 3 : Rat uterus tissue lysate
    Lane 4 : Mouse colon tissue lysate
    Lane 5 : Rat colon tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 90-140 kDa why is the actual band size different from the predicted?



    This data was developed using ab277636, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

     Exposure time: 48 seconds

  • Flow Cytometry - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    Flow Cytometry - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

    This data was developed using ab277636, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MG-63 (human osteosarcoma fibroblast) cells labelling Decorin with ab277636 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

    This data was developed using ab277636, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human esophagus (PMID: 19212830). The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    All lanes : Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

    Lane 1 : Human pancreas tissue lysate
    Lane 2 : HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 40 kDa
    Observed band size: 90-140 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

    Negative control: HT-1080 (PMID:8702652).

    Exposure time: 3 minutes

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

    This data was developed using ab277636, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human bladder cancer. The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    All lanes : Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

    Lane 1 : Human colon tissue treated with PNGase F lysate
    Lane 2 : Human colon tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 90-140 kDa why is the actual band size different from the predicted?



    This data was developed using ab277636, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

    Exposure time: 15 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

    This data was developed using ab277636, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human liver. The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
    Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

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