Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Decorin antibody [EPR24097-105] - BSA and Azide free
See all Decorin primary antibodies
Description

Rabbit monoclonal [EPR24097-105] to Decorin - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, IHC-P, WBmore details
Unsuitable for: ICC or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human colon, uterus, pancreas and placenta tissue lysates; Mouse uterus and colon tissue lysates; Rat uterus and colon tissue lysates. IHC-P: Human colon, esophagus, bladder cancer and liver tissue. Flow cyt: MG-63 cells.
General notes
ab277643 is the carrier-free version of ab277636. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab277643 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24097-105
Isotype

IgG
Research areas

Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

All lanes : Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

Lane 1 : Human uterus tissue lysate
Lane 2 : Human pancreas tissue lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : Human liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

Predicted band size: 40 kDa
Observed band size: 90-140 kDa
why is the actual band size different from the predicted?

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

Lower expression tissue: liver (PMID:1611907).

Exposure time: Lane 1: 8 seconds; Lanes 2-4: 48 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human colon (PMID:32824864). The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

All lanes : Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

Lane 1 : MG-63 (human osteosarcoma fibroblast) whole cell lysate
Lane 2 : Mouse uterus tissue lysate
Lane 3 : Rat uterus tissue lysate
Lane 4 : Mouse colon tissue lysate
Lane 5 : Rat colon tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 40 kDa
Observed band size: 90-140 kDa why is the actual band size different from the predicted?

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

Exposure time: 48 seconds
Flow Cytometry - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MG-63 (human osteosarcoma fibroblast) cells labelling Decorin with ab277636 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human esophagus (PMID: 19212830). The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

All lanes : Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

Lane 1 : Human pancreas tissue lysate
Lane 2 : HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

Predicted band size: 40 kDa
Observed band size: 90-140 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

Negative control: HT-1080 (PMID:8702652).

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human bladder cancer. The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

All lanes : Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

Lane 1 : Human colon tissue treated with PNGase F lysate
Lane 2 : Human colon tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 40 kDa
Observed band size: 90-140 kDa why is the actual band size different from the predicted?

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

Exposure time: 15 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human liver. The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)