Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [106-6] to TMEM119 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Mouse

Overview

  • Product name

    Anti-TMEM119 antibody [106-6] - BSA and Azide free
    See all TMEM119 primary antibodies

  • Description

    Rabbit monoclonal [106-6] to TMEM119 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cytometry: Acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells).

  • General notes

    ab220249 is the carrier-free version of ab210405 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab220249 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Please note that the original Bennett et al . (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author’s permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.

Properties

Images

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210405).

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

    Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells  labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.

    No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).

    The data was provided by Ben Barres’ lab (Stanford University).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210405).

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

    Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (Alexa Fluor® 488). Please refer to ab225497 for protocol details.

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225497 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 488 ab199091 (left). Cells were surface-stained with ab225497, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

    Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (PE). Please refer to ab225496 for protocol details.

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225496 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Phycoerythrin ab209478 (left). Cells were surface-stained with ab225496, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

    Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (Alexa Fluor® 647). Please refer to ab225494 for protocol details.

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225494 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 647 ab199093 (left). Cells were surface-stained with ab225494, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 488 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

  • Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

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