Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3103Y] to Cullin 1/CUL-1
- Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cullin 1/CUL-1 antibody [EPR3103Y]
See all Cullin 1/CUL-1 primary antibodies -
Description
Rabbit monoclonal [EPR3103Y] to Cullin 1/CUL-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB RatHuman -
Immunogen
Synthetic peptide within Human Cullin 1/CUL-1 aa 750 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, MCF7, A549 and PC12 cell lysates; human cervical carcinoma tissue.
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General notes
Previously labelled as Cullin 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3103Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817) at 1/1000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : MDA-MB-435 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817)Immunohistochemical staining of paraffin embedded human lung carcinoma with purified ab75817 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunofluorescence staining of HeLa cells with purified ab75817 at a working dilution of 1/1000, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75817 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cullin 1/CUL-1 with purified ab75817 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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All lanes : Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817) at 1/1000 dilution (purified)
Lane 1 : PC-12 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lane 3 : mouse brain cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
ab75817 (purified) at 1/120 immunoprecipitating Cullin 1 in 10 μg MCF7 whole cell lysate (Lanes 1 and 2, observed at 90 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817)Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab75817 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817)
Unpurified ab75817 at 1/100 dilution staining Cullin 1/CUL-1 in human cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817) at 1/5000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817)
Unpurified ab75817 showing positive staining in Normal human tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817)
Unpurified ab75817 showing positive staining in human Urinary bladder transitional carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817)
Unpurified ab75817 showing positive staining in human Ovarian carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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