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Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3102Y] to Cullin 1/CUL-1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Cullin 1/CUL-1 antibody [EPR3102Y]
    See all Cullin 1/CUL-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3102Y] to Cullin 1/CUL-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cullin 1/CUL-1 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • 293T, A549 and T47D cell lysates; HeLa cells.

  • General notes

     

     

    Previously labelled as Cullin 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)
    Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812) This image is courtesy of an Abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB.

    Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Cullin 1/CUL-1 with ab75812 at 1/400 dilution. Cells were fixed in paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab75812 at 1/400 was carried in PBS buffer for 1 hour at 22°C. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.

    See Abreview

  • Western blot - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)
    Western blot - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)
    All lanes : Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812) at 1/2000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : T47D cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)
    Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)

    ab75812 at 1/100 dilution staining Cullin 1/CUL-1 in HeLa cells by Immunofluorescence.

  • Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)
    Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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