Anti-Cullin 1/CUL-1 antibody (ab225709)
Key features and details
- Rabbit polyclonal to Cullin 1/CUL-1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cullin 1/CUL-1 antibody
See all Cullin 1/CUL-1 primary antibodies -
Description
Rabbit polyclonal to Cullin 1/CUL-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human Cullin 1/CUL-1 aa 726-776. The exact sequence is proprietary. NP_003583.2
Database link: Q13616 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
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General notes
Previously labelled as Cullin 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cullin 1/CUL-1 antibody (ab225709)All lanes : Anti-Cullin 1/CUL-1 antibody (ab225709) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 90 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-Cullin 1/CUL-1 antibody (ab225709)Whole cell lysate from HeLa (human epithelial cell line from cervix adenocarcinoma) cells (1 mg for IP, 20% of IP loaded). ab225709 used for for WB at 0.4 µg/mg and IP at 6 µg/mg lysate.
Lane 1: ab225709 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence 3 seconds.
