Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3103Y] to Cullin 1/CUL-1 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free
    See all Cullin 1/CUL-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3103Y] to Cullin 1/CUL-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, MCF7, A549 and PC12 cell lysates; human cervical carcinoma tissue.

  • General notes

    ab202555 is the carrier-free version of ab75817 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab202555 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Cullin 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)

    Immunofluorescence staining of HeLa cells with purified ab75817 at a working dilution of 1/1000, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75817 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75817).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab75817 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75817).

  • Flow Cytometry - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Flow Cytometry - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cullin 1/CUL-1 with purified ab75817 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75817).

  • Immunoprecipitation - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Immunoprecipitation - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)

    ab75817 (purified) at 1/120 immunoprecipitating Cullin 1 in 10 µg MCF7 whole cell lysate (Lanes 1 and 2, observed at 90 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75817).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Immunohistochemical staining of paraffin embedded human lung carcinoma with purified ab75817 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75817).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)

    Unpurified ab75817 at 1/100 dilution staining Cullin 1/CUL-1 in human cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75817).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)

    Unpurified ab75817 showing positive staining in Normal human tonsil tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75817).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)

    Unpurified ab75817 showing positive staining in human Urinary bladder transitional carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75817).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)

    Unpurified ab75817 showing positive staining in human Ovarian carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75817).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)
    Anti-Cullin 1/CUL-1 antibody [EPR3103Y] - BSA and Azide free (ab202555)

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