Anti-NEK1 antibody (ab229489)
Key features and details
- Rabbit polyclonal to NEK1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-NEK1 antibody
See all NEK1 primary antibodies -
Description
Rabbit polyclonal to NEK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human NEK1 (internal sequence). The exact sequence is proprietary.
Database link: Q96PY6 -
Positive control
- WB: HEK-293T whole cell extracts; mouse brain tissue extract; rat brain tissue extract. ICC/IF: U-87 MG cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-NEK1 antibody (ab229489)Anti-NEK1 antibody (ab229489) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 143 kDa5% SDS-PAGE
-
Western blot - Anti-NEK1 antibody (ab229489)All lanes : Anti-NEK1 antibody (ab229489) at 1/1000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 143 kDa5% SDS-PAGE
-
Immunocytochemistry/ Immunofluorescence - Anti-NEK1 antibody (ab229489)4% paraformaldehyde-fixed U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for NEK1 (green) using ab229489 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
