Anti-Cullin 1/CUL-1 antibody (ab225700)
Key features and details
- Rabbit polyclonal to Cullin 1/CUL-1
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cullin 1/CUL-1 antibody
See all Cullin 1/CUL-1 primary antibodies -
Description
Rabbit polyclonal to Cullin 1/CUL-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP Human
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Immunogen
Synthetic peptide within Human Cullin 1/CUL-1 aa 250-300. The exact sequence is proprietary. (NP_003583.2).
Database link: Q13616 -
Positive control
- IHC-P: Human osteosarcoma tissue. IP: HeLa whole cell lysate.
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General notes
Previously labelled as Cullin 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab225700 was affinity purified using an epitope specific to Cullin 1/CUL-1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 1/CUL-1 antibody (ab225700)
Formalin-fixed, paraffin-embedded human osteosarcoma tissue stained for Cullin 1/CUL-1 using ab225700 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
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Immunoprecipitation - Anti-Cullin 1/CUL-1 antibody (ab225700)Cullin 1/CUL-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225700 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a rabbit anti-Cullin 1/CUL-1 antibody, which recognizes a downstream epitope at 1 µg/ml.
Lane 1: ab225700 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
