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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (ab232532)

Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (ab232532)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13243-53] to ATIC - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-ATIC antibody [EPR13243-53] - BSA and Azide free
    See all ATIC primary antibodies
  • Description

    Rabbit monoclonal [EPR13243-53] to ATIC - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow: HeLa cells.
  • General notes

    Ab232532 is the carrier-free version of ab188321. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232532 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13243-53
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Images

  • Immunoprecipitation - Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (ab232532)
    Immunoprecipitation - Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (ab232532)

    Western blot analysis of ATIC in Jurkat cell lysate immunoprecipitated with ab188321 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Jurkat lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188321).

  • Flow Cytometry - Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (ab232532)
    Flow Cytometry - Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (ab232532)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATIC with ab188321 at 1/140 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188321).

  • Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (ab232532)
    Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (ab232532)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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