Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATIC antibody [EPR13243-53] (ab188321)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13243-53] to ATIC
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ATIC antibody [EPR13243-53]
    See all ATIC primary antibodies

  • Description

    Rabbit monoclonal [EPR13243-53] to ATIC

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, HeLa, HepG2 and HCT-116 cell lysates; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ATIC antibody [EPR13243-53] (ab188321)
    Western blot - Anti-ATIC antibody [EPR13243-53] (ab188321)
    All lanes : Anti-ATIC antibody [EPR13243-53] (ab188321) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HCT-116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

  • Flow Cytometry - Anti-ATIC antibody [EPR13243-53] (ab188321)
    Flow Cytometry - Anti-ATIC antibody [EPR13243-53] (ab188321)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling ATIC with ab188321 at 1/140 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunoprecipitation - Anti-ATIC antibody [EPR13243-53] (ab188321)
    Immunoprecipitation - Anti-ATIC antibody [EPR13243-53] (ab188321)

    Western blot analysis of ATIC in Jurkat cell lysate immunoprecipitated with ab188321 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Jurkat lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-ATIC antibody [EPR13243-53] (ab188321)
    Anti-ATIC antibody [EPR13243-53] (ab188321)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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