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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)

Price and availability

526 012 ₸

Availability

Order now and get it on Friday August 06, 2021

Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13526] to CSL4 - BSA and Azide free
  • Suitable for: IHC-P, IP, ICC, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CSL4 antibody [EPR13526] - BSA and Azide free
    See all CSL4 primary antibodies
  • Description

    Rabbit monoclonal [EPR13526] to CSL4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250355 is the carrier-free version of ab181108.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13526
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Images

  • Western blot - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    Western blot - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    All lanes : Anti-CSL4 antibody [EPR13526] (ab181108) at 1/2000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : Raw264.7 cell lysate
    Lane 4 : PC-12 cell lysate
    Lane 5 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    This data was developed using ab181108, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    This data was developed using ab181108, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix tissue labeling CSL4 with ab181108 at 1/100 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    Immunoprecipitation - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)

    This data was developed using ab181108&

    44; the same antibody clone in a different buffer formulation.

    >Western blot analysis of CSL4 in immunoprecipitation pellets from Raji lysate. ab181108 used at 1/60 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Lane 2 shows negative control.
  • Immunocytochemistry - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    Immunocytochemistry - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    This data was developed using ab181108, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling CSL4 with ab181108 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488), Conterstained with DAPI blue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    This data was developed using ab181108, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CSL4 with ab181108 at 1/100 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    Western blot - Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    All lanes : Anti-CSL4 antibody [EPR13526] (ab181108) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    This data was developed using ab181108, the same antibody clone in a different buffer formulation.

     

  • Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)
    Anti-CSL4 antibody [EPR13526] - BSA and Azide free (ab250355)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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