Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20051] to PEG10/EDR - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free
    See all PEG10/EDR primary antibodies

  • Description

    Rabbit monoclonal [EPR20051] to PEG10/EDR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and HeLa cell lysates.

  • General notes

    ab240392 is the carrier-free version of ab215035 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240392 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as PEG10

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)
    Western blot - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)
    All lanes : Anti-PEG10/EDR antibody [EPR20051] (ab215035) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PEG10 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 30,80 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab215035).

    Lanes 1-3: Merged signal (red and green). Green - ab215035 observed at 100 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab215035 Anti-PEG10/EDR antibody [EPR20051] was shown to specifically react with PEG10/EDR in wild-type HeLa cells. Loss of signal was observed when knockout sample ab258103 was used. Wild-type and PEG10/EDR knockout samples were subjected to SDS-PAGE. ab215035 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on some cells in human placenta is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215035).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on part of the cells in human liver cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215035).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on the germ cells in human testis is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215035).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Negative staining on normal cells in human liver.

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215035).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)
    Immunocytochemistry/ Immunofluorescence - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215035).

  • Flow Cytometry - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)
    Flow Cytometry - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling PEG10/EDR with ab215035 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215035).

  • Immunoprecipitation - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)
    Immunoprecipitation - Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)

    PEG10/EDR was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab215035 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215035 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate, 10 μg (Input).

    Lane 2: ab215035 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215035 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215035).

  • Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)
    Anti-PEG10/EDR antibody [EPR20051] - BSA and Azide free (ab240392)

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