Antibodies, Proteins, Kits and Reagents for Life Science

334 369 ₸ Product size

100 µl 334 369 ₸ 100 µl 334 369 ₸

Order now and get it on Tuesday March 09, 2021

Alexa Fluor® 647 Anti-Histone H3 (di methyl K4) antibody [Y47] (ab200026)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Alexa Fluor® 647 Rabbit monoclonal [Y47] to Histone H3 (di methyl K4)
Suitable for: ICC/IF
Reacts with: Human
Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Product name

Alexa Fluor® 647 Anti-Histone H3 (di methyl K4) antibody [Y47]
See all Histone H3 primary antibodies
Description

Alexa Fluor® 647 Rabbit monoclonal [Y47] to Histone H3 (di methyl K4)
Host species

Rabbit
Conjugation

Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested Applications & Species

Application Species
ICC/IF
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: HeLa cells
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

Y47
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H3 (di methyl K4) antibody [Y47] (ab200026)

ab200026 staining Histone H3 (di methyl K4) in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200026 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
Alexa Fluor® 647 Anti-Histone H3 (di methyl K4) antibody [Y47] (ab200026)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com