Anti-Histone H2B (acetyl K12) antibody - ChIP Grade (ab195494)
Key features and details
- Rabbit polyclonal to Histone H2B (acetyl K12) - ChIP Grade
- Suitable for: ICC/IF, Dot blot, ChIP, WB, ChIP-sequencing
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
-
Product name
Anti-Histone H2B (acetyl K12) antibody - ChIP Grade
See all Histone H2B primary antibodies -
Description
Rabbit polyclonal to Histone H2B (acetyl K12) - ChIP Grade -
Host species
Rabbit -
Specificity
Due to sequence homology we expect this antibody to detect K12 acetylation on multiple H2B variants. -
Tested applications
Suitable for: ICC/IF, Dot blot, ChIP, WB, ChIP-sequencingmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human Histone H2B (acetyl K12) conjugated to keyhole limpet haemocyanin. Peptide surrounding K12.
Database link: Q5QNW6 -
Positive control
- Human HeLa cells and HeLaS3 cells. HeLa whole cell and histone extracts.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
ChIP-sequencing - Anti-Histone H2B (acetyl K12) antibody - ChIP Grade (ab195494)
ChIP-seq analysis using Human HeLaS3 cells, labeling Histone H2B type 1-C/E/F/G/I (acetyl K12) with ab195494 at 0.5 µg and optimized PCR primer sets for qPCR as described above. The 51 bp tags were aligned to the Human genome using the BWA algorithm. The figure shows the enrichment along the complete sequence and a 1 Mb region of the X-chromosome (A and B) and in genomic regions of chromosome 7, surrounding the ACTB gene, and of chromosome 12, surrounding the GAPDH gene (C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.
-
ChIP - Anti-Histone H2B (acetyl K12) antibody - ChIP Grade (ab195494)ChIP analyis using Human HeLa cells, labeling Histone H2B type 1-C/E/F/G/I (acetyl K12) with ab195494 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls, respectively. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
-
Dot Blot - Anti-Histone H2B (acetyl K12) antibody - ChIP Grade (ab195494)Dot Blot analysis of peptides containing the unmodified H2B and other histone modifications, labeling Histone H2B type 1-C/E/F/G/I (acetyl K12) with ab195494 at 1/5000 dilution. 0.2-100 pmol of the peptide containing the respective modifications were spotted onto the membrane.
-
Western blot - Anti-Histone H2B (acetyl K12) antibody - ChIP Grade (ab195494)All lanes : Anti-Histone H2B (acetyl K12) antibody - ChIP Grade (ab195494) at 1/1000 dilution (in TBS-Tween containing 5% skimmed milk)
Lane 1 : HeLa whole cell extracts at 25 µg
Lane 2 : HeLa histone extracts at 15 µg
Lane 3 : Recombinant H2A at 1 µg
Lane 4 : Recombinant H2B at 1 µg
Lane 5 : Recombinant H3 at 1 µg
Lane 6 : Recombinant H4 at 1 µg
Predicted band size: 14 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody - ChIP Grade (ab195494)Immunofluorescent analysis of HeLa cells labeling Histone H2B type 1-C/E/F/G/I (acetyl K12) with ab195494 at 1/500 dilution in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488 (left), DAPI (center) or merged (right). Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA.
