Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade, purified (ab195497)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K9) - ChIP Grade, purified
- Suitable for: Dot blot, ChIP, WB, ICC/IF, ChIP-sequencing
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade, purified
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K9) - ChIP Grade, purified -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, ChIP, WB, ICC/IF, ChIP-sequencingmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (tri methyl K9) conjugated to keyhole limpet haemocyanin.
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Positive control
- ICC/IF: HeLa cells. ChIP: HeLa cells. WB: Histone extracts from HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP-sequencing - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade, purified (ab195497)
ChIP-seq analysis using Human HeLa cells, labeling Histone H3 (tri methyl K9) with ab195497 at 0.5 µg and optimized PCR primer sets for qPCR as described in above. The 36 bp tags were aligned to the human genome using the BWA algorithm. The peak distribution is shown along the long arm of chromosome 19 and a zoomin to an enriched region containing several ZNF repeat genes (A and B) and the enrichment at ZNF510 and ZNF12 on chromosome 9 and 7 respectively (C and D).
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ChIP - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade, purified (ab195497)ChIP analysis using Human HeLa cells, labeling Histone H3 (tri methyl K9) with ab195497 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1000,000 cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the heterochromatin marker Sat2 and for the ZNF510 gene, used as positive controls, and for the promoters of the active c-fos and GAPDH genes, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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ChIP - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade, purified (ab195497)ChIP analysis using HeLa cells, labeling Histone H3 (tri methyl K27) with ab195497. ChIP was performed using sheared chromatin from 1000,000 cells. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoter regions of the active GAPDH and EIF4A2 genes, for the coding region of the ZNF510 gene and for the Sat2 satellite repeat. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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Dot Blot - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade, purified (ab195497)Dot Blot analysis of peptides containing modified and unmodified sequences of histone H3 and H4, labeling Histone H3 (tri methyl K9) with ab195497 at 1/20000 dilution. 0.2-100 pmol of the peptide containing the respective histone modification were spotted onto the membrane.
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Western blot - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade, purified (ab195497)All lanes : Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade, purified (ab195497) at 1/1000 dilution (in TBS-Tween containing 5% skimmed milk)
Lane 1 : Histone extracts from HeLa cells at 15 µg
Lane 2 : Recombinant H3 at 1 µg
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade, purified (ab195497)Immunofluorescent analysis of HeLa cells labeling Histone H3 (tri methyl K9) with ab195497 at 1/500 dilution in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488 (left), DAPI (center) or a merge of the two stainings (right). Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA.
