Anti-AIRE antibody (ab65040)
Key features and details
- Rabbit polyclonal to AIRE
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AIRE antibody
See all AIRE primary antibodies -
Description
Rabbit polyclonal to AIRE -
Host species
Rabbit -
Specificity
ab65040 detects endogenous levels of total AIRE protein. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide: KGRKPPAVPK ALVPPPRLPT KRKASEEARA AAPAALTPRG , corresponding to internal sequence amino acids 111-150 of Human AIRE
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Positive control
- WB: 293 cell extracts. IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab65040 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AIRE antibody (ab65040)ab65040, at a 1/500 dilution, staining AIRE in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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Western blot - Anti-AIRE antibody (ab65040)All lanes : Anti-AIRE antibody (ab65040) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunising peptide at 10 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 58 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
