Anti-Gli2 antibody (ab226390)
Key features and details
- Rabbit polyclonal to Gli2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Gli2 antibody
See all Gli2 primary antibodies -
Description
Rabbit polyclonal to Gli2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human Gli2 aa 950-1000. The exact sequence is proprietary.
Database link: P10070 -
Positive control
- IP: HeLa cell lysate.
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General notes
ab226390 has not performed satisfactorily when used for WB of Gli2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Gli2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Gli2 antibody (ab226390)
ARF6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 0.5 or 1.0 mg for IP, 20% of IP loaded) with ab226390 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226390 at 1 µg/ml.
Lane 1: ab226390 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
