Anti-RON antibody [EPR20953] - BSA and Azide free (ab228855)
![Anti-RON antibody [EPR20953] - BSA and Azide free (ab228855)](https://www.abcam.com/ps/products/228/ab228855/Images/ab228855-3-ab227980293902RON2IHC3.jpg "Anti-RON antibody [EPR20953] - BSA and Azide free (ab228855)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20953] to RON - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-RON antibody [EPR20953] - BSA and Azide free
See all RON primary antibodies -
Description
Rabbit monoclonal [EPR20953] to RON - BSA and Azide free -
Host species
Rabbit -
Specificity
-
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC: Human stomach tissue.
-
General notes
Ab228855 is the carrier-free version of ab227980. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab228855 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20953 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (ab228855)
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous staining on human bladder cancer cells is observed (PMID: 15870710). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227980).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (ab228855)](https://www.abcam.com/ps/products/228/ab228855/Images/ab228855-2-ab227980293901RON2IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (ab228855)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in germinal centers of human tonsil is observed (PMID: 9890710). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227980).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (ab228855)](https://www.abcam.com/ps/products/228/ab228855/Images/ab228855-293905-RON2IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (ab228855)Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in human gastric epithelium is observed (PMID: 9890710). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227980).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-RON antibody [EPR20953] - BSA and Azide free (ab228855)](https://www.abcam.com/ps/products/228/ab228855/Images/ab228855-4-benefits-of-recombinant-antibodies.png)
Anti-RON antibody [EPR20953] - BSA and Azide free (ab228855)
