Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24104-105] to Dicer - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dicer antibody [EPR24104-105] - BSA and Azide free
    See all Dicer primary antibodies

  • Description

    Rabbit monoclonal [EPR24104-105] to Dicer - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt (Intra),ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW264.7, 2.4G2, HeLa, 293T and NIH/3T3, PC-12 lysates. IHC-P: Human testis, Mouse cerebrum and testis, Rat testis tissues.

  • General notes

    ab280957 is the carrier-free version of ab259327. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280957 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)
    Western blot - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)
    All lanes : Anti-Dicer antibody [EPR24104-105] (ab259327) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
    Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : 2.4G2 (rat B cell lymphoma B lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 218 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259327, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: THP-1 (PMID:20584909).

    Lysates were made freshly and used in WB test immediately to minimize protein degradation.

    Exposure time: 48 seconds

     

     

  • Western blot - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)
    Western blot - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)
    All lanes : Anti-Dicer antibody [EPR24104-105] (ab259327) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 218 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    This data was developed using ab259327, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lysates were made freshly and used in WB test immediately to minimize protein degradation.

    Exposure time: Lane 1, 3, 4: 3 minutes

    Lane 2: 26 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)

    This data was developed using ab259327, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis (PMID:17573847). The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)

    This data was developed using ab259327, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID:32737764). The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)

    This data was developed using ab259327, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis. The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)

    This data was developed using ab259327, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis. The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)
    Anti-Dicer antibody [EPR24104-105] - BSA and Azide free (ab280957)

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