Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1132Y] to RON
Suitable for: ELISA, WB, IP, Flow Cyt, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-RON antibody [EP1132Y]
See all RON primary antibodies
Description

Rabbit monoclonal [EP1132Y] to RON
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human RON aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- SKBR3 cell lysate; human stomach tissue.
General notes
The major RON transcript is translated into a glycosylated single chain precursor, cleaved into a 185 kDa heterodimer (p185Ron) of 35 (alpha) and 150 kDa (beta) disulfide-linked chains, before exposure at the cell surface.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1132Y
Isotype

IgG
Research areas

Western blot - Anti-RON antibody [EP1132Y] (ab52927)

Anti-RON antibody [EP1132Y] (ab52927) at 1/10000 dilution + SKBR3 cell lysate at 10 µg

Secondary
HRP-labelled goat anti-rabbit

Predicted band size: 185 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EP1132Y] (ab52927)

Immunohistochemical staining of paraffin-embedded human stomach tissue using ab52927 at a dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-RON antibody [EP1132Y] (ab52927)

Overlay histogram showing THP1 cells stained with ab52927 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52927, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in THP1 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.
ELISA - Anti-RON antibody [EP1132Y] (ab52927)

ELISA analysis of Human RON recombinant protein at 1000 ng/mL with ab52927. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Anti-RON antibody [EP1132Y] (ab52927)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com