Human Stanniocalcin 2 / STC-2 ELISA Kit (ab222880)
Key features and details
- Sensitivity: 0.95 ng/ml
- Range: 1.25 ng/ml - 80 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Stanniocalcin 2 / STC-2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 5.2% Inter-assay Sample n Mean SD CV% Plasma 9.6% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.95 ng/ml -
Range
1.25 ng/ml - 80 ng/ml -
Recovery
96 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Rabbit, Cow, Dog -
Product overview
The Human Stanniocalcin 2 / STC-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222880) is designed for detection of Stanniocalcin 2 / STC-2 in human plasma, serum, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Stanniocalcin 2 / STC-2 in approximately 4 hours. A polyclonal antibody specific for human Stanniocalcin 2 / STC-2 has been pre-coated onto a 96-well microplate with removable strips. Stanniocalcin 2 / STC-2 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human Stanniocalcin 2 / STC-2, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Stanniocalcin 2 / STC-2, also known as stanniocalcin-related protein, is a secreted homodimeric glycoprotein that belongs to the stanniocalcin family. Stanniocalcin 2 / STC-2 contains 302 amino acid residues with 34% STC-1 identity and a molecular mass of 32 kDa. The N terminus of Stanniocalcin 2 / STC-2 contains a signal peptide followed by a cysteine-rich region that may mediate homodimerization. In the kidney, the function of Stanniocalcin 2 / STC-2 on phosphate transport appears to be the opposite to that of STC-1. Stanniocalcin 2 / STC-2 is phosphorylated by casein kinase-2 on serine residues and can inhibit phosphorylation of cell-surface proteins. It is expressed in a wide variety of tissues, including the pancreas, skeletal muscle, and small intestine. Stanniocalcin 2 / STC-2 may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, and cellular calcium/phosphate homeostasis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml Anti- Human Stanniocalcin-2 / STC-2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Human Stanniocalcin-2 / STC-2 antibody 1 x 120µl Chromogen Substrate 1 x 8ml Human Stanniocalcin-2 / STC-2 Standard 1 vial Sealing Tapes 1 x 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Has an anti-hypocalcemic action on calcium and phosphate homeostasis. -
Tissue specificity
Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes. -
Sequence similarities
Belongs to the stanniocalcin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Stanniocalcin 2
- Stanniocalcin related protein
- Stanniocalcin-2
see all -
Database links
- Entrez Gene: 8614 Human
- Omim: 603665 Human
- SwissProt: O76061 Human
- Unigene: 233160 Human
Images
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Human Stanniocalcin 2 / STC-2 standard curve
Example of human Stanniocalcin 2 / STC-2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.