Recombinant human Pyruvate kinase isozyme M1 protein (ab196081)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human Pyruvate kinase isozyme M1 protein -
Biological activity
Specific Activity: ≥54 pmol/min/µg
Assay Conditions: A 50 µl reaction is conducted in a buffer containing 50 mM Tris (pH 7.4), 10 mM MgCl2, 50 mM KCl, 2 mM ADP, 10 mM phosphoenolpyruvate (PEP) at room temperature for 15 min. ATP production is detected using Kinase Assay. -
Purity
>= 80 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICT IGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFAS DPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYME KCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENG GSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASD VHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIE IPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVAN AVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRR LAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPI IAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKAR GFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP -
Predicted molecular weight
59 kDa including tags -
Amino acids
1 to 531 -
Tags
His tag N-Terminus -
Additional sequence information
GenBank Accession No. NM_182470
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 0.45% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Pyruvate kinase isozyme M1 protein (ab196081)
Specific activity of ab196081 was determined to be 54 pmol/min/μg
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SDS-PAGE - Recombinant human Pyruvate kinase isozyme M1 protein (ab196081)SDS-PAGE using 13.5 µg ab196081 (Lane 1). Lane 2 shows protein marker.