HRP Anti-6X His tag® antibody (ab197049)
Key features and details
- HRP Rabbit polyclonal to 6X His tag®
- Suitable for: IHC-P, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
HRP Rabbit polyclonal to 6X His tag® -
Host species
Rabbit -
Conjugation
HRP -
Specificity
ab197049 reacts to recombinant proteins containing the 6X His tag®.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.
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Positive control
- His-tag fusion protein; His-tag protein expressed cell lysate and enriched His-tag protein by IP.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-6X His tag® antibody (ab197049)All lanes : HRP Anti-6X His tag® antibody (ab197049) at 1 µg/ml
Lane 1 : His-tag fusion protein at 5 µg
Lane 2 : His-tag fusion protein at 20 µg
Predicted band size: 50 kDa
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Western blot - HRP Anti-6X His tag® antibody (ab197049)All lanes : HRP Anti-6X His tag® antibody (ab197049) at 1 µg/ml
Lane 1 : His-tag protein in lysate (original cell lysate)
Lane 2 : His-tag protein in lysate (after IP)
Lane 3 : His-tag protein in 100 µL of the IP eluting buffer, enriched with 25 µg of the anti-His tag from 2 mL of the original lysate
Lysates/proteins at 10 µl per lane.
Predicted band size: 50 kDa
