HRP Anti-6X His tag® antibody [4D11] (ab202004)
![HRP Anti-6X His tag® antibody [4D11] (ab202004)](https://www.abcam.com/ps/products/202/ab202004/Images/ab202004-246443-anti-6x-his-tag-antibody-4d11-hrp-western-blot.jpg "HRP Anti-6X His tag® antibody [4D11] (ab202004)")
Key features and details
- HRP Mouse monoclonal [4D11] to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2a
Overview
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Product name
HRP Anti-6X His tag® antibody [4D11]
See all 6X His tag® primary antibodies -
Description
HRP Mouse monoclonal [4D11] to 6X His tag® -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Epitope
This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50). -
Positive control
- WB: E. coli Positive Control (Escherichia coli ) Whole Cell Lysate
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
4D11 -
Myeloma
NS1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-6X His tag® antibody [4D11] (ab202004)HRP Anti-6X His tag® antibody [4D11] (ab202004) at 1/5000 dilution +
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202004 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
