Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µg 321 638 ₸

Order now and get it on Wednesday March 17, 2021

Anti-6X His tag® antibody [4D11] (ab5000)

Key features and details

Mouse monoclonal [4D11] to 6X His tag®
Suitable for: Dot blot, ELISA, WB, IP, ICC/IF, Flow Cyt
Reacts with: Species independent
Isotype: IgG2a

Product name

Anti-6X His tag® antibody [4D11]
See all 6X His tag® primary antibodies
Description

Mouse monoclonal [4D11] to 6X His tag®
Host species

Mouse
Tested applications

Suitable for: Dot blot, ELISA, WB, IP, ICC/IF, Flow Cytmore details
Species reactivity

Reacts with: Species independent
Immunogen

corresponding to 6X His tag®.
Epitope

This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50).
Positive control
- Purchase matching WB positive control:Recombinant ENO1 protein
General notes

This antibody clone is manufactured by Abcam.

HIS-TAG® is a trademark of EMD Biosciences, Inc.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 6.97% L-Arginine, PBS
Concentration information loading...
Purity

IgG fraction
Clonality

Monoclonal
Clone number

4D11
Myeloma

NS1
Isotype

IgG2a
Light chain type

kappa
Research areas

Western blot - Anti-6X His tag® antibody [4D11] (ab5000)

Anti-6X His tag® antibody [4D11] (ab5000) at 1/1000 dilution + 6X His Tagged Protein at 1 µg

Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Exposure time: 30 seconds
Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (ab5000) This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

ab5000 staining Human HEK 293 cells transfected with CACNB3-His by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C. The primary antibody (1/250 in 1% goat serum, 0.1% TX100, 1X PBS) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor^® 546 conjugated goat anti-mouse antibody (1/500) was used as the secondary. DAPI was used for nuclei staining (blue).
See Abreview
Western blot - Anti-6X His tag® antibody [4D11] (ab5000) This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

All lanes : Anti-6X His tag® antibody [4D11] (ab5000) at 1/2000 dilution

Lane 1 : Lysates prepared from HEK-293 cells
Lane 2 : Lysates prepared from HEK-293 cells transfected with Beta3-His (CACNB3)

Secondary
All lanes : HRP-conjugated goat anit-mouse IgG polyclonal at 1/5000 dilution
See Abreview
Immunoprecipitation - Anti-6X His tag® antibody [4D11] (ab5000) This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

ab5000 Immunoprecipitate in human HEK293 whole cell lysate. 25µg of cell lysate incubated with primary antibody (undiluted) and matrix (Protein G) for 16 hours at 4°C. For western blotting an undiluted HRP-Goat anti-rabbit IgG polyclonal was used. Beta3-His fusion protein was immunoprecipitated using anti His Ab, and specific beta3a band (56kDa) was detected using anti beta3 Ab
Lane 1. Lysate of HEK293 cells expressing beta3-His (CACNB3)
Lane 2. IP with anti His Ab
Lane 3. Non bound fraction
See Abreview

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