Antibodies, Proteins, Kits and Reagents for Life Science

Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261)

Key features and details

Biotin Mouse monoclonal [33D10.D2] to 6X His tag®
Suitable for: WB, ELISA
Reacts with: Species independent
Conjugation: Biotin
Isotype: IgG1

Product name

Biotin Anti-6X His tag® antibody [33D10.D2]
See all 6X His tag® primary antibodies
Description

Biotin Mouse monoclonal [33D10.D2] to 6X His tag®
Host species

Mouse
Conjugation

Biotin
Specificity

ab106261 reacts to over-expressed proteins containing the 6X His tag® fused to either the amino or carboxy terminus of transfected or transformed cells. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Tested applications

Suitable for: WB, ELISAmore details
Species reactivity

Reacts with: Species independent
Immunogen

6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
General notes

10-20 BAC molecules per Mouse Monoclonal Antibody

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

33D10.D2
Isotype

IgG1
Research areas

Western blot - Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261)

All lanes : Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261) at 1/5000 dilution (overnight at 4°C)

Lane 1 : Purified histidine-tagged recombinant protein at 0.1 µg
Lane 2 : E. coli cell lysate containing histidine-tagged expression construct at 0.2 µg
Lane 3 : Purified GST-tagged recombinant protein at 0.1 µg
Lane 4 : Purified FLAG-tagged recombinant protein at 0.1 µg

Secondary
All lanes : Peroxidase mouse secondary antibody for 30 minutes at room temperature at 1/20000 dilution

Predicted band size: 1 kDa

Block: 5% non-fat powdered milk for 1 hour at room temperature.
ELISA - Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261)

ELISA analysis of ab106261 Mouse monoclonal [33D10.D2] to 6X His tag® (Biotin).
Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins.
Coating amount: 0.15 µg per welll.
Primary antibody: ab106261 at 100 µg/mL.
Dilution series: 2-fold.
Mid-point concentration: 200 ng/mL.
Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1/10000.
Substrate: TMB ELISA Substrate.

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