Anti-Histone H3 (acetyl K36) antibody (ab232929)
Key features and details
- Rabbit polyclonal to Histone H3 (acetyl K36)
- Suitable for: ChIP-sequencing, WB, ChIP, Dot blot
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
-
Product name
Anti-Histone H3 (acetyl K36) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (acetyl K36) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanChIP-seq HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human Histone H3 (acetyl K36) conjugated to keyhole limpet haemocyanin.
Database link: P68431 -
Positive control
- ChIP: Chromatin from HeLa cells. ChIPseq: Chromatin from HeLa S3 cells. WB: HeLa whole cell and histone extracts.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
ChIP-sequencing - Anti-Histone H3 (acetyl K36) antibody (ab232929)
ChIP was performed on sheared chromatin from 1.5 million HeLa S3 cells using 2 µg ab232929 against Histone H3 (acetyl K36). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the enrichment along the complete sequence and a 1 Mb region of the X-chromosome (A and B) and in genomic regions of chromosome 12 and 3, surrounding the GAPDH and EIF4A2 genes.
-
ChIP - Anti-Histone H3 (acetyl K36) antibody (ab232929)ChIP assays were performed using HeLa (human epithelial cell line from cervix adenocarcinoma) cells, ab232929 against Histone H3 (acetyl K36) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of ab232929 consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the ACTB promoter and for the GAPDH promoter, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
-
Dot Blot - Anti-Histone H3 (acetyl K36) antibody (ab232929)To test the cross reactivity of ab232929 against Histone H3 (acetyl K36), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. ab232929 was used was used at 1/10000 dilution. ab232929 shows a high specificity for the modification of interest.
-
Western blot - Anti-Histone H3 (acetyl K36) antibody (ab232929)All lanes : Anti-Histone H3 (acetyl K36) antibody (ab232929) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 25 µg
Lane 2 : HeLa histone extract at 15 µg
Lane 3 : Recombinant histone H2A at 1 µg
Lane 4 : Recombinant histone H2B at 1 µg
Lane 5 : Recombinant histone H3 at 1 µg
Lane 6 : Recombinant histone H4 at 1 µg
Predicted band size: 15 kDa
