Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

50 µg 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Histone H3 (acetyl K79) antibody [RM156] (ab214731)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [RM156] to Histone H3 (acetyl K79)
Suitable for: ICC/IF, WB, ELISA, ChIP
Reacts with: Human

Product name

Anti-Histone H3 (acetyl K79) antibody [RM156]
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [RM156] to Histone H3 (acetyl K79)
Host species

Rabbit
Specificity

ab214731 reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with other acetylated Lysines in Histone H3.

Tested Applications & Species

Application	Species
ChIP	Human
ELISA	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Histone H3 (acetyl K79). The exact sequence is proprietary.
Positive control
- Acid extracts from HeLa cells treated with sodium butyrate; HeLa whole cell lysate, treated with Sodium Butyrate; HeLa cells with Sodium Butyrate treatment.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

RM156
Isotype

IgG
Research areas

ChIP - Anti-Histone H3 (acetyl K79) antibody [RM156] (ab214731)

ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using ab214731 at 5μg/mL dilution. Real-time PCR was performed using primers specific to the gene indicated.
Western blot - Anti-Histone H3 (acetyl K79) antibody [RM156] (ab214731)

All lanes : Anti-Histone H3 (acetyl K79) antibody [RM156] (ab214731) at 1 µg/ml

Lane 1 : acid extracts from HeLa cells untreated
Lane 2 : acid extracts from HeLa cells treated with sodium butyrate

Predicted band size: 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K79) antibody [RM156] (ab214731)

Immunofluorescent analysis of HeLa cells treated with sodium butyrat labeling Histone H3 (Acetyl-Lys79) with ab214731 at 2 μg/mL dilution (red); actin filaments have been labeled with fluorescein phalloidin (green).
ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (ab214731)

Sandwich ELISA labeling acetylated Histone H3 at Lys 79 in HeLa whole cell lysate, treated or untreated with Sodium Butyrate using ab214731 at 5 μg/mL dilution as the capture antibody and biotinylated anti-H3CT at 1 μg/mL dilution as the detection antibody.
ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (ab214731)

ab214731 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3.
Anti-Histone H3 (acetyl K79) antibody [RM156] (ab214731)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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