Anti-Histone H3 (tri methyl K79) antibody (ab272163)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K79)
- Suitable for: WB, ChIP, ICC/IF, Dot blot
- Reacts with: Mouse, Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K79) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K79) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ChIP, ICC/IF, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Human, Caenorhabditis elegans
Predicted to work with: Rat, Chicken, Drosophila melanogaster
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (tri methyl K79).
Database link: Q71DI3 -
Positive control
- ICC/IF: HeLa cells ChIP: Chromatin prepared from HeLa cells WB: HeLa and NIH/3T3 histone preps; C.elegans embryo lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H3 (tri methyl K79) antibody (ab272163)
Chromatin Immunoprecipitation using ab272163. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 µg of ab272163 and 20 µl of magnetic IgG beads per immunoprecipitation. A "no antibody" (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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Western blot - Anti-Histone H3 (tri methyl K79) antibody (ab272163)All lanes : Anti-Histone H3 (tri methyl K79) antibody (ab272163) at 1 µg/ml
Lane 1 : HeLa human epithelial cell line from cervix adenocarcinoma) histone prep
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) histone prep
Lane 3 : C.elegans embryo lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K79) antibody (ab272163)Immunofluorescence analysis of 0.5% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells during prophase labeling Histone H3 (tri methyl K79) with ab272163 at 1/100 dilution for 1 hour at room temperature, followed by a Dylight 488 secondary antibody at 1/10000 for 45 minutes at room temperature. Histone H3 (tri methyl K79) is green. Nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
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Dot Blot - Anti-Histone H3 (tri methyl K79) antibody (ab272163)Dot Blot of ab272163.
Lane 1: K79 unmodified.
Lane 2: K79me1.
Lane 3: K79me2.
Lane 4: K79me3.
Load: 1, 10, and 100 picomoles of peptide. Primary antibody ab272163 was used at 1/1000 for 45 minutes at 4°C. Followed by the secondary antibody Dylight™488 rabbit at 1/10000 for 45 minutes at room temperature.