Anti-Histone H2B (acetyl K12) antibody (ab61228)
Key features and details
- Rabbit polyclonal to Histone H2B (acetyl K12)
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Histone H2B (acetyl K12) antibody
See all Histone H2B primary antibodies -
Description
Rabbit polyclonal to Histone H2B (acetyl K12) -
Host species
Rabbit -
Specificity
ab61228 detects endogenous levels of Histone H2B only when acetylated at lysine 12. (Human: Lys12; Mouse: Lys12; Rat: Lys12) -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB African green monkey
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Immunogen
Synthetic peptide within Human Histone H2B (acetyl K12). The exact sequence is proprietary.
Sequence:PKK(Ac)GS
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab61228 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody (ab61228)Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Histone H2B (acetyl K12) antibody (ab61228) at 1/50 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide.
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Western blot - Anti-Histone H2B (acetyl K12) antibody (ab61228)All lanes : Anti-Histone H2B (acetyl K12) antibody (ab61228) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cells treated
with TSA (400nM, 24hours), without acetylated peptide
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cells treated
with TSA (400nM, 24hours) with acetylated peptide
Predicted band size: 14 kDa
Observed band size: 14 kDa
