Anti-Histone H3 (phospho T3, di methyl K4) antibody (ab272139)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho T3, di methyl K4)
- Suitable for: Dot blot, ChIP, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (phospho T3, di methyl K4) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (phospho T3, di methyl K4) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, ChIP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (phospho T3, di methyl K4).
Database link: Q71DI3 -
Positive control
- IF: HeLa cells; WB: NIH-3T3 histone prep lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.97% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody (ab272139)
Immunofluorescence analysis of HeLa cells labeling Histone H3 (green) using ab272139 at 1:50, followed by Dylight 488 secondary antibody at 1:10,000. Localization: Histone H3 [p Thr3, Monomethyl Lys4] is nuclear and chromosomal. Countersatin: nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).
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ChIP - Anti-Histone H3 antibody (ab272139)Chromatin Immunoprecipitation of ab272139. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of ab272139 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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Western blot - Anti-Histone H3 antibody (ab272139)Anti-Histone H3 (phospho T3, di methyl K4) antibody (ab272139) at 1/50 dilution + NIH-3T3 histone prep lysate at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDaBlock: 5% BLOTTO
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Dot Blot - Anti-Histone H3 antibody (ab272139)Dot Blot analysis of Histone H3 methylated forms using ab272137 at 1:1000, followed by Dylight 488 secondary antibody at 1:10,000. Load: 1, 10, and 100 picomoles of sample. Block: 5% BLOTTO.
