Anti-acetyl Lysine antibody (ab61257)
Key features and details
- Rabbit polyclonal to acetyl Lysine
- Suitable for: ELISA, IHC-P, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-acetyl Lysine antibody
See all acetyl Lysine primary antibodies -
Description
Rabbit polyclonal to acetyl Lysine -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IHC-P, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic acetylated peptide derived from Lysine-acetlyated proteins (G-A-KA-KA-KA-KA-KA-KA).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab61257 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-acetyl Lysine antibody (ab61257)ab61257 at 1/50 - 1/100 dilution staining Acetyl lysine in human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
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Western blot - Anti-acetyl Lysine antibody (ab61257)All lanes : Anti-acetyl Lysine antibody (ab61257) at 1/500 dilution
Lane 1 : COS7 cell extracts treated
with TSA (400uM, 24hours)
Lane 2 : COS7 cell extracts treated
with TSA (400uM, 24hours) with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Observed band size: 120,25,45,85 kDa why is the actual band size different from the predicted?
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Western blot - Anti-acetyl Lysine antibody (ab61257) Image courtesy of an anonymous Abreview.293 histone (acid extracted) loaded at 2µg.
Blocking step performed using 0.3% BSA for 12 hours at 4°C.ab61257 used at a 1/1000 dilution for 1 hour at 25°C.
The secondary used was an HRP conjugated donkey anti-rabbit IgG polyclonal used at a 1/2000 dilution.
