Anti-hnRNP A2B1 antibody (ab183654)
Key features and details
- Rabbit polyclonal to hnRNP A2B1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-hnRNP A2B1 antibody
See all hnRNP A2B1 primary antibodies -
Description
Rabbit polyclonal to hnRNP A2B1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human hnRNP A2B1 aa 100-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P22626 -
Positive control
- A549, 293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8030 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 and PC-12 whole cell lysates. U373 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-hnRNP A2B1 antibody (ab183654)All lanes : Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : A375 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (ab183654)Immunohistochemical analysis of paraffin-embedded U373 xenograft labeling hnRNP A2B1 with ab183654 at 1/500 dilution.
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Western blot - Anti-hnRNP A2B1 antibody (ab183654)Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution + PC-12 whole cell lysate at 30 µg
Predicted band size: 37 kDa12 % SDS-PAGE
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Western blot - Anti-hnRNP A2B1 antibody (ab183654)All lanes : Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : NIH-3T3 whole cell lysate
Lane 5 : BCL-1 whole cell lysate
Lane 6 : Raw264.7 cell lysate
Lane 7 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa12 % SDS-PAGE
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Immunoprecipitation - Anti-hnRNP A2B1 antibody (ab183654)Western blot analysis using ab183654 at 1/200 dilution detecting hnRNP A2B1 in immunoprecipitation pellets from 4500 µg A549 whole cell lysate with ab183654 at 2 µg. Lane 2 shows Control with 2 μg of preimmune rabbit IgG.
