All lanes : Anti-hnRNP A2B1 antibody (ab227465) at 1/5000 dilution

Lane 1 : Non-transfected A431 (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : hnRNP A2B1-transfected A431 (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts

Lysates/proteins at 30 µg per lane.

Predicted band size: 37 kDa

12% SDS-PAGE gel.

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for hnRNP A2B1 (green) using ab227465 at 1/500 dilution in ICC/IF.

Counterstain: alpha tubulin filaments (red).

Paraffin-embedded human breast cancer tissue stained for hnRNP A2B1 using ab227465 at 1/250 dilution in immunohistochemical analysis.

Anti-hnRNP A2B1 antibody (ab227465) at 1/10000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 37 kDa

12% SDS-PAGE gel.