TCS Antibody Purification Kit - Nanoparticles (ab270554)
Overview
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Product name
TCS Antibody Purification Kit - Nanoparticles -
Product overview
TCS Antibody Purification Kit - Nanoparticles (ab270554) is designed for the purification of IgG fractions from tissue culture supernatants. TCS Protein A resin is prepared by coupling purified Protein A to agarose beads.
The method involves capture of the antibody on the TCS Protein A resin and the removal of unwanted substances using a simple wash procedure. The antibody is then eluted and neutralized. The kit can be used in its rapid version by spin-purification in a centrifuge, or by gravity-flow purification. The antibody to be purified should be in 10 to 50 mL of tissue culture supernatant (TCS). Up to 5 mg of antibody can be purified in each run.
This kit is fully compatible with our nanoparticle conjugation kits (Gold, Latex, Europium and Magnetic) (available separately), which allow the purified antibody to be conjugated rapidly.
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Notes
This product is manufactured by Expedeon, an Abcam company. It was previously called AbPure™ TCS Antibody Purification System. Expedeon product code 264-0030 is the same as the 1 pack size of this product and code 264-0500 is the same as the 3 pack size.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 tests 3 tests ab273955 - Binding Buffer (10X) 1 x 6ml 1 x 18ml ab273967 - Collection Tubes (15 mL) 4 units 12 units ab273953 - Elution Buffer 1 x 7ml 1 x 21ml ab273956 - Neutralizer 1 x 2ml 1 x 6ml ab273964 - Preservative 1 x 2ml 1 x 6ml ab273957 - Protein A resin 1 x 2ml 3 x 2ml ab273914 - Spin Cartridge / Collecting Tube Assemblies 1 unit 3 units ab273966 - Spin Purification Columns 1 unit 3 units ab273954 - Wash Buffer 1 x 25ml 1 x 75ml
Images
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Antibody purification
Purification of Rabbit IgG using ab270554.